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Methods of Bloodcells Counting
Pavlík, Jan ; Provazník, Ivo (referee) ; Hrubeš, Jan (advisor)
At present, the standardization of morphological examination of cytological preparations appears to be very difficult. Digitization and development of computer technology makes it possible to find a suitable solution for the effective processing of cytological samples. Company Cellavision, in collaboration with leading morphologist, developed a device Cellavision DM96, which uses image capture of blood cells for their morphological diagnosis and subsequent archiving. It is of great importance to focus on this system and examine its use in clinical practice. The results obtained by two different methods were statistically processed and evaluated.
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Analysis of cytology images
Pavlík, Jan ; Blaha, Milan (referee) ; Kolář, Radim (advisor)
This master’s thesis is focused on automating the process of differential leukocyte count in peripherial blood using image processing. It deals with the design of the processing of digital images - from scanning and image preprocessing, segmentation nucleus and cytoplasm, feature selection and classifier, including testing on a set of images that were scanned in the context of this work. This work introduces used segmentation methods and classification procedures which separate nucleus and the cytoplasm of leukocytes. A statistical analysis is performed on the basis of these structures. Following adequate statistical parameters, a set of features has been chosen. This data then go through a classification process realized by three artificial neural networks. Overall were classified 5 types of leukocytes: neutropfiles, lymphocytes, monocytes, eosinophiles and basophiles. The sensitivity and specificity of the classification made for 4 out of 5 leukocyte types (neutropfiles, lymphocytes, monocytes, eosinophiles) is higher than 90 %. Sensitivity of classiffication basophiles was evaluated at 75 % and specificity at 67 %. The total ability of classification has been tested on 111 leukocytes and was approximately 91% successful. All algorithms were created in the MATLAB program.
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Methods of Bloodcells Counting
Pavlík, Jan ; Provazník, Ivo (referee) ; Hrubeš, Jan (advisor)
At present, the standardization of morphological examination of cytological preparations appears to be very difficult. Digitization and development of computer technology makes it possible to find a suitable solution for the effective processing of cytological samples. Company Cellavision, in collaboration with leading morphologist, developed a device Cellavision DM96, which uses image capture of blood cells for their morphological diagnosis and subsequent archiving. It is of great importance to focus on this system and examine its use in clinical practice. The results obtained by two different methods were statistically processed and evaluated.
|
|
|
Analysis of cytology images
Pavlík, Jan ; Blaha, Milan (referee) ; Kolář, Radim (advisor)
This master’s thesis is focused on automating the process of differential leukocyte count in peripherial blood using image processing. It deals with the design of the processing of digital images - from scanning and image preprocessing, segmentation nucleus and cytoplasm, feature selection and classifier, including testing on a set of images that were scanned in the context of this work. This work introduces used segmentation methods and classification procedures which separate nucleus and the cytoplasm of leukocytes. A statistical analysis is performed on the basis of these structures. Following adequate statistical parameters, a set of features has been chosen. This data then go through a classification process realized by three artificial neural networks. Overall were classified 5 types of leukocytes: neutropfiles, lymphocytes, monocytes, eosinophiles and basophiles. The sensitivity and specificity of the classification made for 4 out of 5 leukocyte types (neutropfiles, lymphocytes, monocytes, eosinophiles) is higher than 90 %. Sensitivity of classiffication basophiles was evaluated at 75 % and specificity at 67 %. The total ability of classification has been tested on 111 leukocytes and was approximately 91% successful. All algorithms were created in the MATLAB program.
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